Rewiring Translation for Elongation Factor Tu‐Dependent Selenocysteine Incorporation

Abstract

Eine synthetische tRNA (tRNAUTu) wurde als Substrat für drei E.-coli-Proteine verwendet: Seryl-tRNA-Synthetase, die Ser-tRNAUTu bildet, Selenocystein(Sec)-Synthase, die Sec-tRNAUTu erzeugt, und EF-Tu für den Sec-tRNAUTu-Transport zum Ribosom (siehe Schema). tRNAUTu kann vom Ribosom verarbeitet werden, was die ortsspezifische Sec-Insertion in Proteine ermöglicht, darunter Formiat-Dehydrogenase H, Selenoglutaredoxin und Glutathion-Peroxidase. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.