Mechanistic Exploitation of a Self-Repairing, Blocked Proton Transfer Pathway in an O₂-Tolerant [NiFe]-Hydrogenase

Abstract

The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/jacs.8b04798.
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\nDenaturing electrophoresis; plasmid, strains and primer details; PFIRE film preparation; initial E28Q PFE data; additional supporting PFE of E28Q on modified electrodes; PFE data for E28D variant; steady-state solution assay data; X-ray diffraction collection and crystal structure statistics; additional structural figures; PFIRE cyclic voltammograms, chronoamperometry and IR spectra of E28Q (PDF)
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\nja8b04798_si_001.pdf (1.21 MB)