Coupling Amperometry and Total Internal Reflection Fluorescence Microscopy at ITO Surfaces for Monitoring Exocytosis of Single Vesicles

Abstract

Mehr Transparenz in der Bioanalytik: Ein Mikroinstrument bestehend aus transparenten Indiumzinnoxid(ITO)-Elektroden ermöglicht simultane Messungen mittels Amperometrie und Fluoreszenzmikroskopie mit interner Totalreflexion. Die Anwendung dieses Instruments zum gekoppelten optischen und elektrochemischen Nachweis exozytotischer Einzelereignisse wird anhand von enterochromaffinen BON-Zellen demonstriert (siehe Bild). Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.