Proteome map of the chloroplast lumen of Arabidopsis thaliana.

Abstract

The preparation of lumen content fromArabidopsis chloroplasts (page 8355) should be completed as follows. Arabidopsis chloroplasts were prepared from 100 g of leaves (wet weight) as described (1). The leaves were divided in portions of 20 g that were blended five times for 1 s using a Heidolph DIAX 900 homogenizer in 170 ml of 20 mm Tricine-NaOH (pH 8.4), 300 mm sorbitol, 10 mm EDTA, 10 mm KCl, 0.25% (w/v) bovine serum albumin, 4.5 mm sodium ascorbate, and 5 mm cysteine. The preparation was continued as described (1), but the chlo-roplasts were washed and resuspended in 20 mm Hepes-NaOH (pH 7.8), 300 mm sorbitol, 5 mm MgCl2, 2.5 mm EDTA, and 10 mm KCl.