A new nomenclature for the livestock-associated Mycobacterium tuberculosis complex based on phylogenomics

Abstract

<b>Background</b>: <b> </b>The bacteria that compose the <i>Mycobacterium tuberculosis</i> complex (MTBC) cause tuberculosis (TB) in humans and in different animals, including livestock. Much progress has been made in understanding the population structure of the human-adapted members of the MTBC by combining phylogenetics with genomics. Accompanying the discovery of new genetic diversity, a body of operational nomenclature has evolved to assist comparative and molecular epidemiological studies of human TB. By contrast, for the livestock-associated MTBC members, <i>Mycobacterium bovis</i>, <i>M. caprae</i> and <i>M. orygis</i>, there has been a lack of comprehensive nomenclature to accommodate new genetic diversity uncovered by emerging phylogenomic studies. We propose to fill this gap by putting forward a new nomenclature covering the main phylogenetic groups within <i>M. bovis</i>, <i>M. caprae</i> and <i>M. orygis</i>. <b>Methods</b>: <b> </b>We gathered a total of 8,736 whole-genome sequences (WGS) from public sources and 39 newly sequenced strains, and selected a subset of 829 WGS, representative of the worldwide diversity of <i>M. bovis</i>, <i>M. caprae</i> and <i>M. orygis</i>. We used phylogenetics and genetic diversity patterns inferred from WGS to define groups. <b>Results</b>: <b> </b>We propose to divide <i>M. bovis</i>, <i>M. caprae</i> and <i>M. orygis</i> in three main phylogenetic lineages, which we named La1, La2 and La3, respectively. Within La1, we identified several monophyletic groups, which we propose to classify into eight sublineages (La1.1-La1.8). These sublineages differed in geographic distribution, with some being geographically restricted and others globally widespread, suggesting different expansion abilities. To ease molecular characterization of these MTBC groups by the community, we provide phylogenetically informed, single nucleotide polymorphisms that can be used as barcodes for genotyping. These markers were implemented in KvarQ and TB-Profiler, which are platform-independent, open-source tools. <b>Conclusions</b>: <b> </b>Our results contribute to an improved classification of the genetic diversity within the livestock-associated MTBC, which will benefit future molecular epidemiological and evolutionary studies.