Interleukin-35 promotes Breg expansion and interleukin-10 production in CD19+ B cells in patients with ankylosing spondylitis

Abstract

These results demonstrated that reduced IL-35 production may be associated with Bregs defects in AS patients. RIL-35 induced the proliferation of CD19⁺CD24^hiCD38^hi Bregs and IL-10 production, suggesting that IL-35 may serve as a reference for further investigation to develop novel treatments for AS. Key Points • Our study investigated the effects of IL-35 on Bregs in AS patients. • We found the serum IL-35, IL-10 levels, and the percentages of CD19⁺CD24^hiCD38^hi and CD19⁺CD24^hiCD27⁺ Bregs were significantly lower in AS patients. • The serum IL-35 levels were positively correlated with the percentages of CD19⁺CD24^hiCD38^hi and CD19⁺CD24^hiCD27⁺ Bregs in AS patients. • Recombinant IL-35 could effectively promote CD19⁺CD24^hiCD38^hi Breg expansion and IL-10 production.