Antimicrobial Susceptibility of Microbiota in Bacterial Vaginosis Using Fluorescence In Situ Hybridization

Abstract

The probiotic strain <i>Lactobacillus crispatus</i> demonstrated the weakest bacteriolytical effects, followed by metronidazole. Both had no impact on <i>Gardnerella</i> species, instead lysing <i>Prevotella-Bacteroides</i>, <i>Enterobacteriaceae</i> (by <i>L</i>.<i>crispatus</i>) or LGC, <i>Atopobium</i> and <i>Prevotella-Bacteroides</i> (by metronidazole) groups of the microbiota. Cytolytic activity on <i>Gardnerella</i> was highly pronounced and increased from octenisept to ciclopirox, phagolysin, phagolysin with <i>L.crispatus,</i> being best in the combination of phagolysin with <i>S</i>.<i>boulardii</i>. Universally active ciclopirox and octenisept® suppressed nearly all microbial groups including those which are regarded as beneficial. Phagolysin had no effect on naturally occurring <i>Lactobacillus crispatus.</i> Conclusions: FISH susceptibility testing allows unique efficacy evaluation of individually adjusted topical therapy without microbial isolation facilitating optimal therapy choice.