Microfluidic Droplet Cluster with Distributed Evaporation Rates as a Model for Bioaerosols

Abstract

Aerosols and microdroplets are known to act as carriers for pathogens or vessels for chemical reactions. The natural occurrence of evaporation of these droplets has implications for the viability of pathogens or chemical processes. For example, it is important to understand how pathogens survive extreme physiochemical conditions such as confinement and osmotic stress induced by evaporation of aerosol droplets. Previously, larger evaporating droplets were proposed as model systems as the processes in the tiny aerosol droplets are difficult to image. In this context, we propose the concept of evaporation of capillary-clustered aqueous microdroplets dispersed in a thin oil layer. The configuration produces spatially segregated evaporation rates. It allows comparing the consequences of evaporation and its rate for processes occurring in droplets. As a proof of concept, we study the consequences of evaporation and its rate using Escherichia coli (E. coli) and Bacillus subtilis as model organisms. Our experiments indicate that the rate of evaporation of microdroplets is an important parameter in deciding the viability of contained microorganisms. With slow evaporation, E. coli could mitigate the osmotic stress by K+ ion uptake. Our method may also be applicable to other evaporating droplet systems, for example, microdroplet chemistry to understand the implications of evaporation rates.