Supramolecular Fluorescent Probes for the Detection of Reactive Oxygen Species Discovered via High-Throughput Screening

Abstract

Supramolecular fluorescent probes for the detection of reactive oxygen species (ROSs) are designed based on a pro-guest strategy. Nine commercially available fluorescent dyes, six host molecules, and a pro-guest are used to rapidly generate a library of 54 potential supramolecular probes. These potential supramolecular probes are screened in a high-throughput fashion using a plate reader to discover seven "hits" or workable probes. The mechanism is confirmed to be ROS-induced conversion from a low-binding-affinity pro-guest to a high-binding-affinity guest and the competitive displacement of the encapsulated fluorescent dye. The response to H₂O₂ of four supramolecular probes is found to be concentration-dependent and may be used for quantitative analysis of H₂O₂. The supramolecular probe is selectively responsive toward other oxidative agents, such as NaClO and Na₂SO₃. The cell study shows that supramolecular probes are capable of detecting H₂O₂ in human cancer cells (MCF-7 or HeLa).