Molecules · 2022 · 14 citations · 47 references
EngineeringBiochemistryChemistryAnalytical ChemistryBiosensorsSensorsChemical SensorsFluorescence SpectroscopyNanomaterial-based BiosensorsOptical BiosensingOptical BiosensorsOptical SensorsFluorescence BiosensingFluorescence BiosensorsFluorescence Resonance Energy TransferFluorescence-based SensingFluorescent BiosensingFluorescent SensingFluorescent SensorsMethod Detection Limit
An ascorbic acid (AA) sensor was constructed based on the fluorescence resonance energy transfer (FRET) between CdS quantum dots (CdS QDs) and polydopamine (PDA) to detect trace AA sensitively. FRET occurred due to the broad absorption spectrum of PDA completely overlapped with the narrow emission spectrum of CdS QDs. The fluorescence of CdS QDs was quenched and in the “off” state. When AA was present, the conversion of DA to PDA was hindered and the FRET disappeared, resulting in the fluorescence of CdS QDs in an “on” state. Importantly, the degree of fluorescence recovery of CdS QDs displayed a desirable linear correlation with the concentration of AA in the range of 5.0–100.0 μmol/L, the linear equation is y=0.0119cAA+0.3113, and the detection limit is 1.16 μmol/L (S/N = 3, n = 9). There was almost no interference with common amino acid, glucose and biological sulfhydryl small molecules to AA. Trace amount of AA in vitamin C tablets were determined and satisfactory results were obtained; the recoveries were observed to be 98.01–100.7%.
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Polydopamine Spheres as Active Templates for Convenient Synthesis of Various Nanostructures
Jian Yan, Liping Yang, Meng‐Fang Lin et al. · Small · 2012 · 353 citations