Generation of Recombinant Antibodies in HEK293F Cells for the Detection of <i>Staphylococcus aureus</i>

Abstract

<i>Staphylococcus aureus</i> is a major resistant pathogen in clinical practice. Due to the increasing number of infections, rapid and sensitive detection of antibiotic-resistant <i>S. aureus</i> as well as antibiotic-sensitive <i>S. aureus</i> is important for the prevention and control of infectious diseases. In this study, we produced recombinant antibodies against <i>S. aureus</i> from mammalian human embryonic kidney 293 Freestyle cells with high yield and purity. These recombinant antibodies showed high binding affinity and low detection limit in both indirect and sandwich enzyme-linked immunosorbent assays for the detection of methicillin-resistant <i>S. aureus</i> and methicillin-sensitive <i>S. aureus</i>. These results suggest that the recombinant antibodies produced herein can be used for the accurate detection of <i>S. aureus</i> with a wild range of applications in medical diagnosis, food safety, and drug discovery.