ondrion targeting ability and near-infrared emission. NI-VIS possesses a highly sensitive response toward viscosity changes in aqueous environments. As the viscosity of a DPBS-glycerol system increased from 1.0 to 999 cP, NI-VIS exhibited a hundred-fold enhancement in fluorescence. We demonstrated that after the treatment with ionophores, NI-VIS could identify the variation of mitochondrial viscosity in HeLa cells. The probe also recognized the decrease of mitochondria viscosity during starvation-induced mitophagy. More importantly, NI-VIS was successfully applied to visualize the viscosity variation in cirrhotic liver tissues. Our trial with zebrafish suggested this probe could map the microviscosity in vivo. These findings reveal that NI-VIS can serve as a powerful tool to monitor viscosity of biological samples and shows broad potential applications in the biomedical field.20:T4bf,Drug-induced liver injury (DILI) is considered gradually as a serious public health issue, and hepatotoxicity has been regarded as the main clinical problem caused by it. We suspected that both the intracellular viscosity and peroxynitrite (ONOO^-) levels in drug-induced hepatotoxicity tissue are higher than those in a healthy liver. For this reason, we have presented a fluorescent probe VO for multichannel imaging viscosity and ONOO^- simultaneously. Experimental results showed that VO has satisfactory detection performance for both viscosity and ONOO^-, and based on the advantages of its lower cytotoxicity and pH-stabilities, VO was successfully employed to image viscosity and ONOO^- in living cells and animals. More importantly, we use the probe to successfully showcase drug-induced hepatotoxicity by imaging viscosity and ONOO^- induced by acetaminophen (APAP). All the results indicate that VO has great potential for the detection of viscosity and ONOO^- and to assay drug-induced hepatotoxicity. Overall, this work offers a new detection tool/method for a deeper understanding of drug-induced organism injury.

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