Concepedia

3D or not 3D: a guide to assess cell viability in 3D cell systems

Polina Bikmulina, Nastasia V. Kosheleva, Yuri M. Efremov, Artem Antoshin, Zahra Heydari, Valery V. Royuk, Vasily Mikhaylov, Massoud Vosough, Peter Timashev, Yury Rochev,

Soft Matter · 2022 · 33 citations · 33 references

DOI

Concepts

Abstract

The study aims at revealing the influence of particular 3D cell systems’ parameters such as the components’ concentration, gel thickness, cell density, on the cell viability and applicability of standard assays based on different cell properties.

References

33

Substrate Compliance versus Ligand Density in Cell on Gel Responses

Adam J. Engler, Lucie Bačáková, Cynthia Newman et al. · Biophysical Journal · 2004 · 1.1K citations · Full text

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Preparation of ready-to-use, storable and reconstituted type I collagen from rat tail tendon for tissue engineering applications

Navneeta Rajan, Jason Habermehl, Marie‐France Côté et al. · Nature Protocols · 2006 · 579 citations

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The use of Alamar Blue assay for quantitative analysis of viability, migration and invasion of choriocarcinoma cells

Salwan Al‐Nasiry, Nele Geusens, M. Hanssens et al. · Human Reproduction · 2007 · 486 citations · Full text

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Print Me An Organ! Why We Are Not There Yet

Wei Long Ng, Chee Kai Chua, Yufang Shen · Progress in Polymer Science · 2019 · 281 citations · Full text

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Cell viability assessment using the Alamar blue assay: A comparison of 2D and 3D cell culture models

Franck Bonnier, Mark Keating, Tomasz P. Wróbel et al. · Toxicology in Vitro · 2014 · 271 citations · Full text

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