Genome-Wide Identification and Functions against Tomato Spotted Wilt Tospovirus of PR-10 in Solanum lycopersicum

Abstract

Tomato spotted wilt virus impacts negatively on a wide range of economically important plants, especially tomatoes. When plants facing any pathogen attack or infection, increase the transcription level of plant genes that are produced pathogenesis-related (PR) proteins. The aim of this study is a genome-wide identification of PR-10 superfamily and comparative analysis of <i>PR-10</i> and <i>Sw-5b</i> gene functions against tomato responses to biotic stress (TSWV) to systemic resistance in tomato. Forty-five candidate genes were identified, with a length of 64-210 amino acid residues and a molecular weight of 7.6-24.4 kDa. The <i>PR-10</i> gene was found on ten of the twelve chromosomes, and it was determined through a genetic ontology that they were involved in six biological processes and molecular activities, and nine cellular components. Analysis of the transcription level of PR-10 family members showed that the <i>PR-10</i> gene (Solyc09g090980) has high expression levels in some parts of the tomato plant. <i>PR-10</i> and <i>Sw-5b</i> gene transcription and activity in tomato leaves were strongly induced by TSWV infection, whereas H8 plants having the highest significantly upregulated expression of <i>PR-10</i> and <i>Sw-5b</i> gene after the inoculation of TSWV, and TSWV inoculated in M82 plants showed significantly upregulated expression of <i>PR-10</i> gene comparatively lower than H8 plants. There was no significant expression of <i>Sw-5b</i> gene of TSWV inoculated in M82 plants and then showed highly significant correlations between <i>PR-10</i> and <i>Sw-5b</i> genes at different time points in H8 plants showed significant correlations compared to M82 plants after the inoculation of TSWV; a heat map showed that these two genes may also participate in regulating the defense response after the inoculation of TSWV in tomato.