Effect of α-Tocopherol Deficiency on Carp-III

Abstract

Quantitative and qualitative gas chromatography of triglycerides has been extended to fish lipids containing both odd and even carbon number fatty acids. A 50cm short glass column containing 2% OV-17 on 80/100 mesh Shimalite W gave good resolution of trigly-ceride peaks at a higher temperature, 280°-340°C. Qualitative analysis was found to be possible by using calibration factors determined by the internal normalization technique. The triglycerides of two fish oils (carp and rainbow trout), soybean oil and cod liver oil were analysed by this method. This method was also useful for determining the triglyceride compositions of other fish oils.