Nitrite- and N2O-reducing bacteria respond differently to ecological factors in saline lakes

Abstract

The distribution of nitrite- and N2O-reducing bacteria is key to potential N2O emission from lakes. However, such information in highland saline lakes remains unknown. Here, we investigated the abundance and community composition of nitrite- and N2O-reducing bacteria in the sediments of six saline lakes on the Qing-Tibetan Plateau. The studied lakes covered a wide range of salinity (1.0-340.0 g/L). Results showed that in the studied saline lake sediments, nitrite-reducing bacteria were significantly more abundant than N2O-reducing bacteria, and their abundances ranged 7.14 × 103-8.26 × 108 and 1.18 × 106-6.51 × 107 copies per gram sediment (dry weight), respectively. Nitrite-reducing bacteria were mainly affiliated with α-, β- and γ-Proteobacteria, with β- and α-Proteobacteria being dominant in low- and high-salinity lakes, respectively; N2O-reducing bacterial communities mainly consisted of Proteobacteria (α-, β-, γ- and δ-subgroups), Bacteroidetes, Verrucomicrobia, Actinobacteria, Chloroflexi, Gemmatimonadetes and Balneolaeota, with Proteobacteria and Bacteroidetes/Verrucomicrobia dominating in low- and high-salinity lakes, respectively. The nitrite- and N2O-reducing bacterial communities showed distinct responses to ecological factors, and they were mainly regulated by mineralogical and physicochemical factors, respectively. In response to salinity change, the community composition of nitrite-reducing bacteria was more stable than that of N2O-reducing bacteria. These findings suggest that nitrite- and N2O-reducing bacteria may prefer niches with different salinity.