The nano-CuCl₂.2H₂O salt was prepared by the ball milling method. The association parameters for bulk and nano-CuCl₂ salts in H₂O are estimated at different temperatures using the conductivity method by applying the Fuoss-Shedlovsky equation and it was higher for nano-CuCl₂ than bulk CuCl₂ salt. The interaction between the cation (Cu²⁺) and ligand (ceftriaxone) in H₂O was determined also by the conductometric method. Two stoichiometric complexes 1/2 and 1/1 (M/L) are estimated and follow the order K _f (1/1) > K _f (1 : 2) and ∆G _f (1/1) > ∆G _f (1/2) for (M : L) (in negative values) indicate the favorable of formation of (1/1) complex compared to the (1 : 2) complex. The Gibbs free energies change was increased in negative signs with increasing the temperature. The antimicrobial activities of CFT, bulk Cu-CFT complex, and nano-Cu-CFT complex were studied on LB agar by the disc diffusion technique against clinical isolates of gram-negative bacteria (Klebsiella pneumonia and Pseudomonas aeruginosa) and Fungi (Candida albicans). It was observed that (CFT) has a higher zone of inhibition and antibacterial activity than that of bulk and nano-Cu-CFT complexes in Klebsiella pneumonia and Pseudomonas aeruginosa (gram-negative bacteria). The nano-Cu-CFT complex showed a higher clear zone of inhibition and antifungal activity against candida than bulk Cu-CFT complex while the absence of the inhibition zone in CFT, so nano-Cu-CFT complex, can be used as an antifungal drug.

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