One-step light-up metabolic probes for <i>in situ</i> discrimination and killing of intracellular bacteria

Abstract

TPEPy-Ala and TPAPy-Kdo with metabolic moieties can be directly incorporated into the bacterial cell envelopes and light up intracellular bacteria. Additionally, the metabolic probes can effectively eliminate labeled bacteria in situ with minimal host cell cytotoxicity via photodyanmic therapy.