Lactiplantibacillus plantarum AR113 Exhibit Accelerated Liver Regeneration by Regulating Gut Microbiota and Plasma Glycerophospholipid

Abstract

Emerging evidence indicates that probiotics have been proved to influence liver injury and regeneration. In the present study, the effects of <i>Lactiplantibacillus plantarum</i> AR113 on the liver regeneration were investigated in 70% partial hepatectomy (PHx) rats. Sprague-Dawley (SD) rats were gavaged with <i>L. plantarum</i> AR113 suspensions (1 × 10¹⁰ CFU/mL) both before and after partial hepatectomy. The results showed that <i>L. plantarum</i> AR113 administration 2 weeks before partial hepatectomy can accelerate liver regeneration by increased hepatocyte proliferation and tumor necrosis factor-α (TNF-α), hepatocyte growth factor (HGF), and transforming growth factor-β (TGF-β) expression. Probiotic administration enriched <i>Lactobacillus</i> and <i>Bacteroides</i> and depleted <i>Flavonifractor</i> and <i>Acetatifactor</i> in the gut microbiome. Meanwhile, <i>L. plantarum</i> AR113 showed decline of phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidyl serine (PS), and lysophosphatidyl choline (LysoPC) levels in the serum of the rats after the <i>L. plantarum</i> AR113 administration. Moreover, <i>L. plantarum</i> AR113 treated rats exhibited higher concentrations of L-leucine, L-isoleucine, mevalonic acid, and lower 7-oxo-8-amino-nonanoic acid in plasma than that in PHx. Spearman correlation analysis revealed a significant correlation between changes in gut microbiota composition and glycerophospholipid. These results indicate that <i>L. plantarum</i> AR113 is promising for accelerating liver regeneration and provide new insights regarding the correlations among the microbiome, the metabolome, and liver regeneration.