Nano Letters · 2021 · 49 citations · 39 references
MedicineBioengineeringNuclear MedicineBiomedical ImagingHybrid ImagingMolecular ImagingEnzyme ImagingFluorescence ImagingMolecular Probe DevelopmentPositron Emission TomographyTheranostic NanoparticlesTumor ImagingActivatable Imaging ProbesEnzyme-activated Imaging ProbesFluorescence Imaging ProbesIn Vivo Fluorescence ImagingIntraoperative ImagingMolecular Imaging ProbesNanoparticlesNear-infrared Fluorescence Imaging
Smart near-infrared (NIR) fluorescence (FL) and positron emission tomography (PET) bimodal probes have shown promise for preoperative and intraoperative imaging of tumors. In this paper, we report an enzyme-activatable probe (P-CyFF-68Ga) and its cold probe (P-CyFF-Ga) using an enzyme-induced fluorogenic reaction and in situ coassembly strategy and demonstrate the utility for NIR FL/PET bimodality imaging of enzymatic activity. P-CyFF-68Ga and P-CyFF-Ga can be converted into dephosphorylated CyFF-68Ga and CyFF-Ga in response to alkaline phosphatase (ALP) and subsequently coassemble into fluorescent and radioactive nanoparticles (NP-68Ga). The ALP-triggered in situ formed NP-68Ga is prone to anchoring on the ALP-positive HeLa cell membrane, permitting the concurrent enrichment of NIR FL and radioactivity. The enhancements in NIR FL and radioactivity enables high sensitivity and deep-tissue imaging of ALP activity, consequently facilitating the delineation of HeLa tumor foci from the normal tissues in vivo.
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Molecular optical imaging probes for early diagnosis of drug-induced acute kidney injury
Jiaguo Huang, Yan Lyu, Qingqing Miao et al. · Nature Materials · 2019 · 708 citations
Medicine, Nephrology, Pharmacology +16