<I>In vitro</I> and <I>in vivo</I> effects of short-term cold storage of platelets in PAS-C

Abstract

In vitro and in vivo effects of short-term cold storage of platelets in PAS-C Cold (4C)-stored platelets (CSP) were the standard of care in the 1960s and 1970s but fell out of favor when their short in vivo survival was discovered. Since then, room temperature-stored platelets (RSP) have been the standard of care. Septic transfusion reactions from bacterially contaminated RSP remain the most common transfusiontransmitted infection. In addition, accumulating data questioning the efficacy and safety of RSP, together with a short shelf life, highlight an unmet medical need for an alternative product. Currently, CSP are being re-evaluated for bleeding patients, for whom immediate function matters more than long circulation time. An added benefit of CSP is that bacterial growth is markedly reduced at 4C. However, how to store CSP best is poorly understood. Some groups reported increased wastage due to aggregates in CSP stored in plasma. 3]