Contributions of polysaccharides to arsenate resistance in Chlamydomonas reinhardtii

Abstract

Polysaccharides supply energy for various metabolic processes in cells. However, their roles in the arsenate (As(V)) resistance in microalgae remain largely unknown. Here, we explored the synthesis and transformation of polysaccharides in Chlamydomonas reinhardtii upon various levels of As(V) stress, using a number of physiological indexes along with transmission electron microscopic (TEM) and proteomic analyses. When exposed to low concentration of As(V) (0-20 μg/L), C. reinhardtii accumulated starch and produced more extracellular polysaccharides. At 50 μg/L As(V) treatment, starch accumulation gradually shifted to polysaccharides decomposition in the algal cells. Under higher As(V) concentration (500 μg/L), significantly more proteins in fatty acid metabolic pathway were differentially expressed, indicating that cells redirected carbon flux and transformed lipids into polysaccharides. The findings of this study demonstrate that polysaccharides may be critically involved in the As(V) resistance of C. reinhardtii.