Transport of organic compounds in the mammal. Partition of dieldrin and telodrin between the cellular components and soluble proteins of blood

Abstract

2. Mannono-(1-+5)-lactone caused 50 % inhibi- tion of the hydrolysis of 6 mM-p-nitrophenyl a-mannoside at 0-080 mm by a rat-epididymal preparation and at 0x098 mm by a limpet prepara- tion.The concentration for 50 % inhibition of the hydrolysis of 2*5 mM-phenyl ,B-mannoside by the limpet preparation was 0-013 mi.3. Concentrations of mannono-(1--4)-lactone causing 50 % inhibition under the same conditions were 40 mm for rat-epididymal a-mannosidase, 26 mm for limpet oc-mannosidase and 7-1 mm for limpet ,-mannosidase.4. Both mannonolactones acted competitively on rat-epididymal ac-mannosidase, with values for Ki of 32 and 0-071 mm for the (1-*4)-and the (1-÷5)-lactone respectively.5. Glucono-(1--*5)-lactone caused 50 % inhibi- tion of the hydrolysis of 6 mM-p-nitrophenyl oc-mannoside at 8-2 mm by the rat-epididymal preparation, and of 5 mM-phenyl a-glucoside at 0-85 mm by the same preparation.With the limpet preparation the concentrations for 50 % inhibition of the hydrolysis of 5 mM-o-nitrophenyl ,B-glucoside and 5 mM-phenyl a-glucoside were 0-092 and 2-2 mm respectively.6. Concentrations of glucono -(1-4) -lactone causing 50 % inhibition under the same conditions were 23 mm for rat-epididymal oc-mannosidase, 1-3 mm for epididymal oc-glucosidase, 0-24 mm for limpet P-glucosidase and 3-2 mm for limpet ac-glucosidase.7. Neither mannono-( 1-4)nor mannono-(l1-5)- lactone caused any inhibition of the limpet /1- glucosidase, and they were alike in their very feeble inhibitory power towards rat-epididymal a-glucosidase.