Engineered Alkane‐Hydroxylating Cytochrome P450_BM3 Exhibiting Nativelike Catalytic Properties

Abstract

Divide, evolve, and conquer: A domain-based strategy (see scheme) was used to engineer high catalytic and coupling efficiency for propane hydroxylation in a multidomain cytochrome P450 enzyme. The engineered enzymes exhibit high total activities in whole-cell bioconversions of propane to propanol under mild conditions, using air as oxidant.