Engineered Alkane‐Hydroxylating Cytochrome P450_BM3 Exhibiting Nativelike Catalytic Properties

Abstract

Divide, evolve, and conquer: A domain-based strategy (see scheme) was used to engineer high catalytic and coupling efficiency for propane hydroxylation in a multidomain cytochrome P450 enzyme. The engineered enzymes exhibit high total activities in whole-cell bioconversions of propane to propanol under mild conditions, using air as oxidant. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z702616_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.