Genome-Wide Identification, Characterization and Expression Analysis of Soybean CHYR Gene Family

Abstract

The <i>CHYR</i> (CHY ZINC-FINGER AND RING FINGER PROTEIN) proteins have been functionally characterized in iron regulation and stress response in <i>Arabidopsis</i>, rice and <i>Populus</i>. However, their roles in soybean have not yet been systematically investigated. Here, in this study, 16 <i>GmCHYR</i> genes with conserved Zinc_ribbon, CHY zinc finger and Ring finger domains were obtained and divided into three groups. Moreover, additional 2-3 hemerythrin domains could be found in the N terminus of Group III. Phylogenetic and homology analysis of <i>CHYRs</i> in green plants indicated that three groups might originate from different ancestors. Expectedly, <i>GmCHYR</i> genes shared similar conserved domains/motifs distribution within the same group. Gene expression analysis uncovered their special expression patterns in different soybean tissues/organs and under various abiotic stresses. Group I and II members were mainly involved in salt and alkaline stresses. The expression of Group III members was induced/repressed by dehydration, salt and alkaline stresses, indicating their diverse roles in response to abiotic stress. In conclusion, our work will benefit for further revealing the biological roles of <i>GmCHYRs</i>.