An Activatable Afterglow/MRI Bimodal Nanoprobe with Fast Response to H₂S for In Vivo Imaging of Acute Hepatitis

Abstract

Accurate detection of hepatic hydrogen sulfide (H₂ S) to monitor H₂ S-related enzymes' activity is critical for acute hepatitis diagnosis, but remains a challenge due to the dynamic and transient nature of H₂ S. Here, we report a H₂ S-activatable near-infrared afterglow/MRI bimodal probe F1-GdNP, which shows an "always-on" MRI signal and "off-on" afterglow signal toward H₂ S. F1-GdNP shows fast response, high sensitivity and specificity toward H₂ S, permitting afterglow imaging of H₂ S and evaluation of cystathionine γ-lyase (CSE)'s activity in living mice. We further employ the high spatial-resolution MRI signal of F1-GdNP to track its delivery and accumulation in liver. Importantly, F1-GdNP offers a high signal-to-background ratio (SBR=86.2±12.0) to sensitively report on the increased hepatic H₂ S level in the acute hepatitis mice via afterglow imaging, which correlated well with the upregulated CSE activity in the liver, showcasing the good potential of F1-GdNP for monitoring of acute hepatitis process in vivo.