Divide and Be Conquered—Cell Cycle Reactivation in Arbuscular Mycorrhizal Symbiosis

Abstract

Arbuscular mycorrhizas (AM) are widespread symbiotic associations between 72% of plant species (Genre et al., 2020) and soilborne Glomeromycotina fungi (Spatafora et al., 2016). Analogous associations with fungi appeared over 400 million years ago (Strullu-Derrien et al., 2014; and are thought to have played a major role in the transition of plants from aquatic to terrestrial environments (Wang et al., 2010).Indeed, extant AM fungi supply their host plants with water and mineral nutrients, resources that are as indispensable for them as they were for their earliest ancestors (Feijen et al., 2018). AM development is a conserved process across extant host species with limited morphological variations in the symbiotic structures (Choi et al., 2018). Following an exchange of chemical signals (Choi et al., 2018) root colonization starts with the formation of a hyphopodium on the root surface (Kobae et al., 2018).Fungal entry in epidermal cells is anticipated by the assembly of the prepenetration apparatus (PPA), a broad, nucleus-associated cytoplasmic bridge. Here, exocytic and endocytic processes (Genre et al., 2005(Genre et al., , 2008Russo et al., 2019b) contribute to build a novel cell compartment, the symbiotic interface, hosting hyphae within an invagination of the plant cell membrane and a layer of unstructured cell wall components (Parniske, 2008;Balestrini and Bonfante, 2014). Such Symbiotic interfaces accommodate all intracellular hyphae as they develop towards the inner cortex, where their repeated branching originates arbuscules: the distinctive structures of this symbiosis, where mineral nutrients and water are transferred to the plant across the extensive periarbuscular interface (Luginbuehl and Oldroyd, 2017), in exchange for sugars and lipids (Choi et al., 2018).The study of fungal accommodation has revealed that host cell rearrangement and major changes in gene expression extend to neighbouring, uncolonized cells (Genre et al., 2008;Pumplin and Harrison, 2009;Gaude et al., 2012), indicating that signalling processes anticipate fungal development within the root tissues. In this context, we have recently shown that such prepenetration responses include cell cycle reactivation in cortical cells, with anticlinal cell divisions and recursive endoreduplication anticipating fungal colonization (Russo et al., 2019 a,b;Carotenuto et al., 2019 a,b). We here propose a model depicting the recruitment of cell cycle processes as a strategy for arbuscule accommodation, speculating on its conservation in other, more recent, biotrophic interactions.Microscopy, flow cytometry and gene expression studies have revealed the activation of cell divisionrelated processes since the early steps of AM development, often at a distance from the colonizing hyphae (Russo et al., 2019a,b;Carotenuto et al., 2019a,b). Firstly, the presence of sparse couples of 'split-cells' in the inner root cortex was consistently observed in both young and fully developed colonization units from diverse plants . Such split-cells were half the length of the surrounding parenchymal cells, suggesting the occurrence of cell division after tissue differentiation. This was confirmed by the direct observation of dividing cells as early as 48 hours post hyphopodium formation in Daucus carota expressing a GFP fusion with TPLATE , an adaptin-related protein that accumulates on the cell plate membrane and plasmalemma at the cortical division zone (Van Damme et al., 2006). Furthermore, combined flow cytometry and confocal imaging allowed the precise localization of inner cortical cells with different levels of increased ploidy in the AM colonized areas .This revealed the diffuse occurrence of endoreduplication events -i.e. DNA duplication in the absence of cell division (Barow, 2006) -throughout AM development (Carotenuto et al., 2019b), as supported by the upregulation of several key endocycle and S-phase marker genes . In addition, uncolonized split cells often displayed lower ploidy than their neighbouring undivided cells, suggesting that cell division and endoreduplication combine to generate the resulting mixed population of cells with diverse ploidy levels (Carotenuto et al., 2019b).These observations outlined a previously unpredicted scenario of cell cycle reactivation in response to AM colonization. Attempting to explain the origin and role of these ubiquitous but histologically localized responses, a few additional considerations should be discussed.Firstly, cell divisions in the inner cortex have been observed when intraradical hyphae were limited to epidermal and outer cortical layers but not in later stages; by contrast, recursive endoreduplication cycles appear to be active for a longer period of time, with arbusculated and neighbouring cells reaching levels of 128 and even 256C ploidy, corresponding to up to 6 cycles of endoreduplication. In more detail, endoreduplication appeared to surge at the front of fungal expansion and reach the highest peaks in the central area of infection units, suggesting the existence of a proportion between ploidy and the abundance (or age) of intraradical fungal structures. Importantly, Carotenuto et al (2019b) also observed that the couples of 'split' cortical cells derived from cell division often displayed different ploidy levels, with higher ploidy in cells that were closer to the fungus or hosting an older arbuscule. This confirms that cell division takes place before endoreduplication, or at least that endoreduplication can proceed after cell division.Secondly, the observation of both cell division and ploidy increase at a distance from arbuscules or colonizing hyphae suggests the existence of a yet unidentified signalling process reactivating the cell cycle before fungal arrival.In addition, the concentration of both ectopic cell divisions and endocycle events to the inner cortex envisages a remarkable correlation with the accommodation of arbuscules, which normally develop in the same cell layer. Cell proliferation, with its limited occurrence, appears to have a secondary role, if any, in the generation of additional space for arbuscule accommodation. By contrast, the sparse cell divisions observed in AM colonized areas might relate to the developmental fate of cortical cells. In the roots of most plants, in fact, cortical cell differentiation is determined with an endocycle that doubles their DNA content from 2C to 4C (Cebolla, 1999;Edgar et al., 2014) with a consequent size increase (Robinson et al., 2018). In line with that, in situ studies of cell ploidy in uncolonized roots of M. truncatula revealed that most cortical cells had 4C nuclei, while a few of them displayed 8C and 16C ploidy levels. Even if experimentally challenging, it would be very interesting to investigate if there is a relationship between initial cell ploidy and the occurrence of ectopic cell division in early AM interaction.Besides tissue differentiation, endoreduplication is also common in plant interactions with diverse microbes: replicating DNA produces multiple copies of each gene, intensifying cell responsiveness to microbial signals. Examples are numerous, from pathogens and parasites (de Almeida-Engler and Gheysen, 2013; Chandran et al., 2016;Wildermuth et al., 2017) to symbionts (Suzaki et al., 2014;Lace & Ott, 2018). Furthermore, endoreduplication-related cell enlargement is typically associated to the accommodation of several microbes, and specifically to arbuscules in AM (Balestrini and Bonfante, 2014;Heck et al., 2016).The requirement of a specific rearrangement in the host cell organization for arbuscule accommodation is apparent from a simple observation of the structural and functional complexity of the periarbuscular interface (Luginbuhel and Oldroyd 2017; Choi et al., 2018;Roth et al., 2019;Ivanov et al., 2019), compared to the tunnel-like interface hosting linear hyphae in outer root tissues. In fact, while epidermal and outer cortical PPAs are structured as roughly linear cytoplasmic bridges across the vacuole, the PPAs that generate periarbuscular interfaces are much more complex and extensive, appearing as large accumulations of cytoplasm that extend from the hyphal penetration site and occupy most of the host cell central volume (Genre et al., 2008). Such a massive, centripetally-oriented exocytic event has striking ultrastructural and molecular similarities with the assembly of the cell plate on the cell equatorial plane at the end of mitosis, and indeed an evolutionary correlation between symbiotic interface biogenesis and cell plate deposition has been envisaged in both AM (Russo et al., 2019b) and N-fixing nodulation (Downie, 2014). In support of this hypothesis, in vivo imaging of GFP-TPLATE fusions revealed a strong accumulation of TPLATE at sites of PPA assembly and at sites of cell-to-cell hyphal passage, where the perifungal membrane fuses with the plasmalemma in striking analogy with cell plate fusion with the cell membrane at the end of mitosis (Russo et al., 2019b).If the recruitment of cell division processes to assemble the extensive symbiotic interface surrounding each arbuscule now appears more convincing, developmental restraints could contribute to explain why sparse cell division and diffuse endoreduplication are limited to the cortex. Dong et al. (2021) have recently highlighted that a SHR-SCR module (known to regulate cortex/endodermis initial cell division in the root meristem) maintains its activity and is required for cell cycle reactivation in legume inner cortex during nodule organogenesis (Suzaki et al., 2014). Even if analogous studies in rice (which does not form root nodules, but hosts AM fungi) did not confirm SHR-SCR expression in cortical cells, it is reasonable to speculate that analogous mechanisms involving meristematic transcription factors maintain a disposition to reactivate the cell cycle in inner corticals. This peculiarity is likely related to the evolution of root branching (Xiao et al., 2019), but appears to have later been co-opted in several plant interactions, from N-fixing symbioses (Dong et al., 2021) to nematode parasitism (de Almeida-Engler and Gheysen, 2013), where both cell division and endoreduplication are required for microbe accommodation. While anyway such processes involve the formation of new organs (i.e. lateral roots, N-fixing nodules or nematode-hosting cysts), their occurrence in AM, where organogenesis is absent, appears puzzling; even more so if we consider that AM symbiosis appeared in land plants before the evolution of true roots (Strullu-Derrien et al., 2014;.By discussing the developmental and evolutionary context where cell cycle processes interweave with AM symbiosis, a scenario emerges (Fig. 1) where the perception of AM fungal colonization in outer root tissues triggers a so-far unknown intraradical signalling process activating cell cycle-related processes ahead of the penetrating intraradical hyphae. Inner cortical cells, due to their peculiar developmental status deploy two downstream responses: a few of them (possibly depending on their ploidy) complete mitosis, splitting in two smaller cells, as cell elongation is very limited in a mature tissue (Russo et al., 2019a, b); the remaining majority of inner corticals enter the endocycle, duplicating their DNA content up to several times, continuously stimulated by the approaching fungal symbiont -in fact endoreduplication also extends to those cells that had divided earlier (Carotenuto et al., 2019a, b). Such a model implies that host cell largely anticipate and direct fungal colonization, in line with previous propositions that the plant holds substantial control over symbiosis development (Parniske, 2008).We currently have no information on how the earliest land plants acquired the ability to host a symbiotic fungus inside their cells. One can speculate that initial surface interactions provided an advantageous exchange of nutrients, pressing towards more intimate contacts, such as the penetration of fungal hyphae between the plant cells and eventually inside their lumen. In this context, creating de novo a fully functional symbiotic interface -as in modern plants -appears unrealistic. By contrast, stimulating cell divisions in differentiated organs could have been a more amenable strategy to generate both crack openings in the surface tissues (an entry route that is conserved in many extant plant-microbe interactions; Ibáñez et al., 2017) and irregular intercellular spaces in the inner ones, producing a protected niche for the fungus. The subsequent re-routing of cell plate formation towards the creation of a more efficient symbiotic interface appears achievable, especially in the light of the current findings, and the observation of split-cells in some of the earliest fossils of AM hosts indicates that this is indeed an ancient response associated with fungal accommodation (Strullu-Derrien et al., 2018).