Yersiniabactin contributes to overcoming zinc restriction during <i>Yersinia pestis</i> infection of mammalian and insect hosts

Abstract

<i>Yersinia pestis</i> causes human plague and colonizes both a mammalian host and a flea vector during its transmission cycle. A key barrier to bacterial infection is the host's ability to actively sequester key biometals (e.g., iron, zinc, and manganese) required for bacterial growth. This is referred to as nutritional immunity. Mechanisms to overcome nutritional immunity are essential virulence factors for bacterial pathogens. <i>Y. pestis</i> produces an iron-scavenging siderophore called yersiniabactin (Ybt) that is required to overcome iron-mediated nutritional immunity and cause lethal infection. Recently, Ybt has been shown to bind to zinc, and in the absence of the zinc transporter ZnuABC, Ybt improves <i>Y. pestis</i> growth in zinc-limited medium. These data suggest that, in addition to iron acquisition, Ybt may also contribute to overcoming zinc-mediated nutritional immunity. To test this hypothesis, we used a mouse model defective in iron-mediated nutritional immunity to demonstrate that Ybt contributes to virulence in an iron-independent manner. Furthermore, using a combination of bacterial mutants and mice defective in zinc-mediated nutritional immunity, we identified calprotectin as the primary barrier for <i>Y. pestis</i> to acquire zinc during infection and that <i>Y. pestis</i> uses Ybt to compete with calprotectin for zinc. Finally, we discovered that <i>Y. pestis</i> encounters zinc limitation within the flea midgut, and Ybt contributes to overcoming this limitation. Together, these results demonstrate that Ybt is a bona fide zinc acquisition mechanism used by <i>Y. pestis</i> to surmount zinc limitation during the infection of both the mammalian and insect hosts.