Cytological and molecular characteristics of delayed spike development in wheat under low temperature in early spring

Abstract

Low temperature in early spring impairs wheat growth and grain yield. However, little is known about the cytological and molecular mechanisms underlying low temperature regulation of wheat spike development. Microstructure observation and transcriptome sequencing of wheat spikes under low temperature were conducted. Low temperature slowed spike development, reduced the yield-component parameters of wheat spikes at the harvest stage, delayed the formation of lateral spikelets and tissue development, and induced the early differentiation of terminal spikelets. Low temperature increased the content of abscisic acid and caused the upregulation of genes in the abscisic acid signaling pathway, including those encoding PP2Cs, SnRK2s, and bZIP transcription factors. Low temperature also induced the upregulation of 33 cold-responsive genes involved in wheat response to low-temperature stress and regulation of abscisic acid biosynthesis and metabolism of other substances. The wheat spike adapted to cold conditions by changing the expression levels of genes involved in spike morphogenesis, including the transcription-factor genes MADS6, ERF4, ERF78, WOX6, and NAC48. These findings suggest that low temperature in early spring delays wheat spike development by increasing abscisic acid content or affecting the expression of genes involved in morphogenesis.