Exploring the five-paced viper (Deinagkistrodon acutus) venom proteome by integrating a combinatorial peptide ligand library approach with shotgun LC-MS/MS

Abstract

These findings demonstrated that the CPLL enrichment method worked well in finding the trace toxin proteins in <i>D. acutus</i> venom, in contrast with the previous venomic characterization of <i>D. acutus</i> by conventional LC-MS/MS. In conclusion, this approach combined with the CPLL enrichment was effective for allowing us to explore the hidden <i>D. acutus</i> venomic profile and extended the list of potential venom toxins.