The m⁶A reader MhYTP2 regulates <i>MdMLO19</i> mRNA stability and antioxidant genes translation efficiency conferring powdery mildew resistance in apple

Abstract

N⁶ -methyladenosine (m⁶ A) reader protein plays an important role in trichome morphology, developmental timing and morphogenesis in Arabidopsis. However, the function of m⁶ A readers in plant-microbe interaction remains unclear. Here, a Malus YTH-domain family protein MhYTP2 was initially characterized as an m⁶ A reader. MhYTP2 overexpression increased mRNA m⁶ A modification level and translation efficiency. The m⁶ A in the exon regions appeared to destabilize the mRNAs, whereas m⁶ A in the untranslated regions positively correlated with the associated mRNA abundance. MhYTP2 overexpression enhanced apple powdery mildew resistance, possibly by rapidly degrading the bound mRNAs of MdMLO19 and MdMLO19-X1 and improving the translation efficiency of the antioxidant genes. To conclude, the results shed light on the apple m⁶ A profile, the effect of MhYTP2 on m⁶ A profile, and the m⁶ A roles in MdMLO19 and MdMLO19-X1 mRNAs stability and glutamate dehydrogenase 1-like MdGDH1L mRNA translation efficiency.