Nitrogen-Doped Carbon Dot and CdTe Quantum Dot Dual-Color Multifunctional Fluorescent Sensing Platform: Sensing Behavior and Glucose and pH Detection

Abstract

A novel fluorescent probe based on a nitrogen-doped carbon dot (N-CD) and CdTe quantum dot (CdTe QD) platform has been constructed for H₂O₂/glucose detection and pH sensing. In this work, H₂O₂-tolerant blue fluorescence N-CDs were added to the H₂O₂-mediated yellow fluorescence quenching of CdTe QDs to construct a dual-color ratiometric fluorescent H₂O₂ probe. H₂O₂-induced passivated group detachment and action on deep nanocrystals promoted CdTe QD fluorescence quenching. Meanwhile, the addition of the blue fluorescent background of N-CDs sharply reflected the color change in CdTe QDs. Under the optimized experimental conditions, the platform was effectively applied to the detection of H₂O₂ produced by the enzymatic reaction of glucose, showing high sensitivity (limit of detection 7.86 μM) and wide linear range (26-900 μM) for glucose detection. The pH-sensing behavior of CdTe QDs and N-CDs was attributed to the displacement of a weak acid (3-mercaptopropionic acid) by a strong acid (HCl) and the acid titration process of two coexisting bases (N-CDs and NH₃·H₂O), respectively. The loss of passivation and doping effects led to a decrease in the fluorescence intensity of CdTe QDs and N-CDs. Moreover, utilizing the ability of bimaterial system fluorescence to pH sensing, a semiquantitative pH detection based on the linear response was developed. The pH range was analyzed by three kinds of N-CD (<i>F</i>_ex = 440 nm) and CdTe QD (<i>F</i>_ex = 548 nm) typical emission spectral shapes. In addition, the recovery results showed that the bimaterial system was proved to be appropriate for the assay of glucose in spiked serum samples.