Synthesis titanium dioxide nanoparticles doped with silver and Novel antibacterial activity

Abstract

Abstract Pure and Ag doped TiO 2 nanoparticles were synthesis via a hydrothermal process and have anti-bacterial activity were examined against Escherichia coli, Staphylococcus aureus. The effect of Ag doping on TiO 2 to rutile phase transformation was investigated by X-ray diffraction (XRD), Scanning electron microscopy (SEM),(EDX), Fourier transform infrared (FTIR) spectroscopy, and (UV). The average size of the TiO 2 was in the range of (12.6)nm, and the doped TiO 2 +Ag had an average size of (12)nm. The antibacterial activity of the Tio 2 and TiO 2 +Ag were evaluated against E. coli,S. aureus using the release of cellular materials method. The results showed that TiO 2 +Ag nanoparticles as a novel DNA-mediated antibacterial agent. The TiO 2 +Ag nanoparticles were observed to destroy the bacterial cells by permeating the bacterial nucleic acid and cytoplasmic membrane, resulting in the loss of cell wall integrity, nucleic acid damage, and increased cell-wall permeability.