Morphology and Phylogeny of Gnomoniopsis (Gnomoniaceae, Diaporthales) from Fagaceae Leaves in China

Abstract

<i>Gnomoniopsis</i> (<i>Gnomoniaceae</i>, <i>Diaporthales</i>) is a well-classified genus inhabiting leaves, branches and fruits of the hosts in three plant families, namely <i>Fagaceae</i>, <i>Onagraceae</i> and <i>Rosaceae</i>. In the present study, eighteen <i>Gnomoniopsis</i> isolates were obtained from diseased leaves of <i>Fagaceae</i> hosts collected from Fujian, Guangdong, Hainan, Henan, Jiangxi and Shaanxi provinces in China. Morphology from the cultures and phylogeny based on the 5.8S nuclear ribosomal DNA gene with the two flanking internally transcribed spacer (ITS) regions, the translation elongation factor 1-alpha (<i>tef1</i>) and the beta-tubulin (<i>tub2</i>) genes were employed to identify these isolates. As a result, seven species were revealed, viz. <i>Gnomoniopsis</i>&nbsp;<i>castanopsidis</i>, <i>G.</i>&nbsp;<i>fagacearum</i>, <i>G</i>. <i>guangdongensis</i>, <i>G.</i>&nbsp;<i>hainanensis</i>, <i>G</i>. <i>rossmaniae</i> and <i>G</i>. <i>silvicola</i> spp. nov, as well as a known species <i>G. daii</i>. In addition, <i>G. daii</i> was firstly reported on the host <i>Quercus</i>&nbsp;<i>aliena</i>.