Paraburkholderia dioscoreae sp. nov., a novel plant associated growth promotor

Abstract

A novel bacterium, designated strain Msb3^T, was recently isolated from leaves of the yam family plant <i>Dioscorea bulbifera</i> (<i>Dioscoreaceae</i>). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that this strain belonged to the genus <i>Paraburkholderia</i> with <i>Paraburkholderia xenovorans</i> as nearest validly named neighbour taxon (99.3 % sequence similarity towards the <i>P. xenovorans</i> type strain). Earlier genome sequence analysis revealed a genome of 8.35 Mb in size with a G+C content of 62.5 mol%, which was distributed over two chromosomes and three plasmids. Here, we confirm that strain Msb3^T represents a novel <i>Paraburkholderia</i> species. <i>In silico</i> DNA-DNA hybridization and average nucleotide identity (OrthoANIu) analyses towards <i>P. xenovorans</i> LB400^T yielded 58.4 % dDDH and 94.5 % orthoANIu. Phenotypic and metabolic characterization revealed growth at 15 °C on tryptic soy agar, growth in the presence of 1 % NaCl and the lack of assimilation of phenylacetic acid as distinctive features. Together, these data demonstrate that strain Msb3^T represents a novel species of the genus <i>Paraburkholderia,</i> for which we propose the name <i>Paraburkholderia dioscoreae</i> sp. nov. The type strain is Msb3^T (=LMG 31881^T, DSM 111632^T, CECT 30342^T).