High-throughput fast full-color digital pathology based on Fourier\n ptychographic microscopy via color transfer

Abstract

Full-color imaging is significant in digital pathology. Compared with a\ngrayscale image or a pseudo-color image that only contains the contrast\ninformation, it can identify and detect the target object better with color\ntexture information. Fourier ptychographic microscopy (FPM) is a\nhigh-throughput computational imaging technique that breaks the tradeoff\nbetween high resolution (HR) and large field-of-view (FOV), which eliminates\nthe artifacts of scanning and stitching in digital pathology and improves its\nimaging efficiency. However, the conventional full-color digital pathology\nbased on FPM is still time-consuming due to the repeated experiments with\ntri-wavelengths. A color transfer FPM approach, termed CFPM was reported. The\ncolor texture information of a low resolution (LR) full-color pathologic image\nis directly transferred to the HR grayscale FPM image captured by only a single\nwavelength. The color space of FPM based on the standard CIE-XYZ color model\nand display based on the standard RGB (sRGB) color space were established.\nDifferent FPM colorization schemes were analyzed and compared with thirty\ndifferent biological samples. The average root-mean-square error (RMSE) of the\nconventional method and CFPM compared with the ground truth is 5.3% and 5.7%,\nrespectively. Therefore, the acquisition time is significantly reduced by 2/3\nwith the sacrifice of precision of only 0.4%. And CFPM method is also\ncompatible with advanced fast FPM approaches to reduce computation time\nfurther.\n