Biomolecular NMR Assignments · 2021 · 10 citations · 50 references
Life SciencesMicrobiologyMolecular BiologyGenomicsMolecular VirologyVirologyRna StructureUntranslated RegionsViral Genome OrganizationViral Regulatory ElementsViral Rna BiologyViral StructureChemical Shift AnalysisRna Internal LoopsStem-loop StructureStem-loopsSubgenomic Messenger RnaSubgenomic Viral RnaViral Rna Cis-regulatory ElementsViral Rna Structure
Abstract The stem-loop (SL1) is the 5'-terminal structural element within the single-stranded SARS-CoV-2 RNA genome. It is formed by nucleotides 7–33 and consists of two short helical segments interrupted by an asymmetric internal loop. This architecture is conserved among Betacoronaviruses. SL1 is present in genomic SARS-CoV-2 RNA as well as in all subgenomic mRNA species produced by the virus during replication, thus representing a ubiquitous cis -regulatory RNA with potential functions at all stages of the viral life cycle. We present here the 1 H, 13 C and 15 N chemical shift assignment of the 29 nucleotides-RNA construct 5_SL1, which denotes the native 27mer SL1 stabilized by an additional terminal G-C base-pair.
50