Vitamin D Inhibits IL-22 Production Through a Repressive Vitamin D Response Element in the il22 Promoter

Abstract

Th22 cells constitute a recently described CD4⁺ T cell subset defined by its production of interleukin (IL)-22. The action of IL-22 is mainly restricted to epithelial cells. IL-22 enhances keratinocyte proliferation but inhibits their differentiation and maturation. Dysregulated IL-22 production has been associated to some inflammatory skin diseases such as atopic dermatitis and psoriasis. How IL-22 production is regulated in human T cells is not fully known. In the present study, we identified conditions to generate Th22 cells that do not co-produce IL-17 from naïve human CD4⁺ T cells. We show that in addition to the transcription factors AhR and RORγt, the active form of vitamin D₃ (1,25(OH)₂D₃) regulates IL-22 production in these cells. By studying T cells with a mutated vitamin D receptor (VDR), we demonstrate that the 1,25(OH)₂D₃-induced inhibition of <i>il22</i> gene transcription is dependent on the transcriptional activity of the VDR in the T cells. Finally, we identified a vitamin D response element (VDRE) in the <i>il22</i> promoter and demonstrate that 1,25(OH)₂D₃-VDR directly inhibits IL-22 production <i>via</i> this repressive VDRE.