Turning waste into wealth: facile and green synthesis of carbon nanodots from pollutants and applications to bioimaging

Abstract

In an effort to turn waste into wealth, Reactive Red 2 (RR2), a common and refractory organic pollutant in industrial wastewater, has been employed for the first time as a precursor to synthesize carbon nanodots (CNDs) by a facile, green and low-cost route, without utilization of any strong acids or other oxidizers. The detailed characterizations have confirmed that the synthesized CNDs exhibit good water dispersibility, with a mean particle size of 2.43 nm and thickness of 1-3 layers. Importantly, the excellent fluorescence properties and much reduced biotoxicity of the CNDs confer its potential applications in further biological imaging, which has been successfully verified in both <i>in vitro</i> (cell culture) and <i>in vivo</i> (zebrafish) model systems. Thus, it is demonstrated that the synthesized CNDs exhibit nice biocompatibility and fluorescence properties for bioimaging. This work not only provides a novel economical and environmentally friendly approach in recycling a chemical pollutant, but also greatly promotes the potential application of CNDs in biological imaging.