Near-infrared spectroscopy as a tool for rapid screening of deoxynivalenol in wheat flour and its applicability in the industry

Abstract

This study aimed to evaluate the applicability and efficiency of Near-Infrared Spectroscopy (NIR) by using dispersive NIR and Fourier Transform NIR to analyse 267 samples of Brazilian wheat flour contaminated with deoxynivalenol (DON). For this, Partial Least-squares Discriminant Analysis (PLS-DA) and Principal Component Analysis-Linear Discriminant Analysis (PC-LDA) were used as discriminatory methods. Next, the samples were classified according to the maximum tolerated limits (MTL) for DON in Brazil, 750 μg kg^-1, and two groups were established for the calibration set: category A (≤450 μg kg^-1), non-contaminated or below the MTL; and category B (>450 μg kg^-1), contaminated or above the MTL. Validation samples through PLS-DA showed correct classification rates in the range of 85-87.5% and presented a 10-15% error; for PC-LDA, the hit rate was over 85% with an error of 10-15%. The present findings demonstrate that NIR is an excellent alternative method to classify wheat flour samples according to DON content.