Electrospun Nanofibers with High Specific Surface Area to Prepare Modified Electrodes for Electrochemiluminescence Detection of Azithromycin

Abstract

Polyacrylonitrile (PAN) and (CH3COO)2Zn were used as raw materials, and carbon nanofibers (CNFs) with high specific surface area were successfully prepared by an electrospinning method. A new method of electrochemiluminescence detection of azithromycin was established by modifying the glassy carbon electrode (GCE). Under the optimal conditions, the electrochemical behavior and electrochemiluminescence behavior of the Ru(bpy)32+-AZM system on the modified electrode were investigated. Owing to the large specific area, more active sites, and promotion of electron transfer, the sensor exhibits high electrocatalytic activity, high sensitivity, a good linear relationship ranging from <a:math xmlns:a="http://www.w3.org/1998/Math/MathML" id="M1"> <a:mn>8.0</a:mn> <a:mo>×</a:mo> <a:msup> <a:mrow> <a:mn>10</a:mn> </a:mrow> <a:mrow> <a:mo>−</a:mo> <a:mn>8</a:mn> </a:mrow> </a:msup> </a:math> to <c:math xmlns:c="http://www.w3.org/1998/Math/MathML" id="M2"> <c:mn>1.0</c:mn> <c:mo>×</c:mo> <c:msup> <c:mrow> <c:mn>10</c:mn> </c:mrow> <c:mrow> <c:mo>−</c:mo> <c:mn>4</c:mn> </c:mrow> </c:msup> </c:math> mol/L, and a low detection limit ( <e:math xmlns:e="http://www.w3.org/1998/Math/MathML" id="M3"> <e:mn>6.52</e:mn> <e:mo>×</e:mo> <e:msup> <e:mrow> <e:mn>10</e:mn> </e:mrow> <e:mrow> <e:mo>−</e:mo> <e:mn>8</e:mn> </e:mrow> </e:msup> </e:math> mol/L). In addition, the good recoveries indicate that the sensor was a promising device for the detection of azithromycin in real samples.