31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016): part one

Abstract

Here we describe a novel method utilizing phylogenetic and sequencing analysis to visualize and quantify TCR repertoire diversity. To demonstrate the utility of the approach, we have applied it to understanding the shaping of the CD8 T Cell response to self (Kb-TRP2) and foreign (Kb-SIY) antigens in nave and tumor bearing B6 mice. Additionally, this method was applied to tumor infiltrating lymphocytes (TIL's) from patients undergoing Nivolumab (anti-PD-1) therapy in a clinical trial for metastatic melanoma to understand TCR repertoire characteristics between responders and non-responders.