Bimetallic Metal–Organic Frameworks: Enhanced Peroxidase-like Activities for the Self-Activated Cascade Reaction

Abstract

Metal-organic frameworks (MOFs) are significant useful molecular materials as a result of their high surface area and flexible catalytic activities by tuning the metal centers and ligands. MOFs have attracted great attention as efficient nanozymes recently; however, it is still difficult to understand polymetallic MOFs for enzymatic catalysis because of their complicated structure and interactions. Herein, bimetallic NiFe₂ MOF octahedra were well prepared and exhibited enhanced peroxidase-like activities. The synergistic effect of Fe and Ni atoms was systematically investigated by electrochemistry, X-ray photoelectron spectrometry, (XPS) and <i>in situ</i> Raman techniques. The electrons tend to transfer from Ni²⁺ to Fe³⁺ in NiFe₂ MOFs, and the resulting Fe²⁺ is ready to decompose H₂O₂ and generate ^·OH by a Fenton-like reaction. After integration with glucose oxidase (GOx), which can downgrade the pH value and generate H₂O₂ by oxidation of glucose, a self-activated cascade reagent is therefore established for efficiently inducing cell death. The changes of cell morphology, DNA, and protein are also successfully recorded during the cell death process by Raman spectroscopy and fluorescence imaging.