Transposon Tn554 encodes three products required for transposition.

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

Life Sciences, Microbiology, Molecular Biology +10

Detection of specific sequences among DNA fragments separated by gel electrophoresis

E.M. Southern · Journal of Molecular Biology · 1975 · 32.8K citations

Life Sciences, Molecular Biology, Molecular Genetics +12

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I

Peter Rigby, Marianne Dieckmann, Carl Rhodes et al. · Journal of Molecular Biology · 1977 · 11.7K citations

Life Sciences, Molecular Biology, Molecular Genetics +14

A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments

Joachim Messing, Jeffrey Vieira · Gene · 1982 · 2.9K citations

Life Sciences, Biotechnology, Molecular Biology +9

Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.

Mark D. Biggin, Toby J. Gibson, G. F. Hong · Proceedings of the National Academy of Sciences · 1983 · 2.4K citations · Full text

Life Sciences, Biochemistry, Molecular Biology +11