High-performance thin layer chromatography: A powerful analytical technique in pharmaceutical drug discovery

Abstract

HPTLC is an advanced type of planar chromatography used extensively in the recent years for fingerprinting of medicinal plants, products and for screening lichen substances, quantification of active ingredients and herbal drugs, phytochemical and biomedical analysis and also used to check of presence of adulterants in the formulation. It is more sensitive and possible to run more sample in a short period of time, by using small amount of solvent. It is one of the sophisticated instrumental techniques based on the full capabilities of thin layer chromatography. In this paper, a brief discussion of the type of instrument used in HPTLC, its entire approach, and how this procedure is superior to TLC is presented. This article attempts to concentrate on the use of HPTLC by including examples of medications, medicinal drugs, and formulations that have been analyzed using HPTLC.