Bee Pollen Polysaccharide From Rosa rugosa Thunb. (Rosaceae) Promotes Pancreatic β-Cell Proliferation and Insulin Secretion

Abstract

Insufficient pancreatic β-cell or insulin-producing β-cell are implicated in all types of diabetes mellitus. Our previous studies showed bee pollen polysaccharide RBPP-P improves insulin resistance in type 2 diabetic mice by inhibiting liver fat deposition. However, its potential of regulating β-cell function and integrity is not fully known. Herein, we observed that β-cell proliferation (<i>n</i> = 10), insulin synthesis (<i>n</i> = 5, <i>p =</i> 0.01684) and insulin incretion (<i>n</i> = 5, <i>p =</i> 0.02115) were intensely activated in MIN6 cells when treatment with RBPP-P. In alloxan-induced diabetic mice, oral administration of RBPP-P (<i>n</i> = 10) effectively decreased the blood glucose (<i>p =</i> 0.0326), drink intake (<i>p</i> < 0.001) and urine (<i>p</i> < 0.001). It directly stimulated phosphorylation of p38 (<i>p =</i> 0.00439), ERK (<i>p =</i> 0.02951) and AKT (<i>p =</i> 0.0072) to maintain the islet function and mass. Thus, our data suggest that RBPP-P is a natural compound to regulate β-cell proliferation and function, indicating it might have therapeutic potential against type 1 diabetes.