<i>YKT6</i> , as a potential predictor of prognosis and immunotherapy response for oral squamous cell carcinoma, is related to cell invasion, metastasis, and CD8+ T cell infiltration

Abstract

Metastasis and immune suppression account for the poor prognosis of oral squamous cell carcinoma (OSCC). <i>YKT6</i> is a member of the soluble NSF attachment protein receptor (SNARE) family, and the effect of <i>YKT6</i> in OSCC remains elusive. The purpose of this study was to explore promising prognostic and immune therapeutic candidate biomarkers for OSCC and to understand the expression pattern, prognostic value, immune effects, and biological functions of <i>YKT6</i>. Genes correlated with tumor metastasis and CD8 + T cell levels were identified by weighted gene coexpression network analysis (WGCNA). Next, <i>YKT6</i> was analyzed through differential expression, prognostic and machine learning analyses. The molecular and immune characteristics of <i>YKT6</i> were analyzed in independent cohorts, clinical specimens, and <i>in vitro</i>. In addition, we investigated the role of <i>YKT6</i> at the pan-cancer level. The results suggested that the red module in WGCNA, as a hub module, was associated with lymph node (LN) metastasis and CD8 + T cell infiltration. Upregulation of <i>YKT6</i> was found in OSCC and linked to adverse prognosis. A nomogram model containing <i>YKT6</i> expression and tumor stage was constructed for clinical practice. The aggressive and immune-inhibitory phenotypes showed <i>YKT6</i> overexpression, and the effect of <i>YKT6</i> on OSCC cell invasion and metastasis <i>in vitro</i> was observed. Moreover, the low expression of <i>YKT6</i> was correlated with high CD8 + T cell levels and potential immunotherapy response in OSCC. Similar results were found at the pan-cancer level. In total, <i>YKT6</i> is a promising candidate biomarker for prognosis, molecular, and immune characteristics in OSCC.