Chemical Communications · 2021 · 14 citations · 31 references
Natural SciencesBiophysicsCell BiologyBiomedical ImagingBiophotonicsMolecular ImagingCellular ImagingFluorescence ImagingFluorescence MicroscopyMolecular Probe DevelopmentPhotophysics Of Fluorescent ProbesSuper-resolution MicroscopyFluorophore DesignLive-cell Fluorescence ImagingLive-cell Imaging ProbesMicroscopyMicroscopy ImagingSuper-resolutionSuper-resolution Fluorescence ImagingSuper-resolution Fluorescence Microscopy
Fluorophores with photo-modulatory fluorescence properties are valuable for cutting-edge localization microscopy. The existing probes are either photo-activatable, or photo-switchable, but not both. We report a probe (DH-SiR), a leuco-dye obtained by reduction of Si-rhodamine, with both photo-activatable and photo-switchable fluorescence. The potential for super-resolution microscopy was showcased.
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Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
Eric Betzig, George H. Patterson, Rachid Sougrat et al. · Science · 2006 · 8.9K citations