Designing a cross-linked redox network for a mediated enzyme-based electrode

Abstract

A bio-conjugated redox network matrix based on glucose dehydrogenase, thionine (diamine-containing mediator), and poly(ethylene glycol) diglycidyl ether (crosslinker) is developed on a glassy carbon electrode through covalent bonding with one-pot crosslinking. Electrons from the enzyme diffuse through the network producing 400 μA cm-2 of glucose oxidation current at 25 °C.