Synadenium grantii Hook f.: HPLC/QTOF-MS/MS TENTATIVE IDENTIFICATION OF THE PHYTOCONSTITUENTS, ANTIOXIDANT, ANTIMICROBIAL AND ANTIBIOFILM EVALUATION OF THE AERIAL PARTS

Abstract

A rapid and simple effective technique HPLC/QTOF-MS/MS was developed to identify the constituents in synadenium grantii. This study aims to investigate antioxidant, antimicrobial and antibiofilm of aqueous methanol extract. Adequate conditions for extraction were employed and LC-MS/MS analysis was optimized in positive ion mode using full scan MS measurements and MS/MS fragmentations. The high concentration of S. grantiie extract exhibited a promising radical scavenging activity with all the tested antioxidant assays. It found to be 82.400.24, 75.690.30 and 90.270.16% using DPPH, Ion metal chelating and ABTS radical scavenging activity respectively, while it found to be 1.410.02 Reducing power capacity and 2787 27.1molTrolox/100 g D.W. (Ferric reducing power). The antioxidant activity of the extract was found to be as near as that of the synthetic BHA. Biofilm is a specific polymeric matrix produced by several bacterial species to make it more resistant against the immune system and diverse antibiotics. A total of 84 different metabolites were tentatively identified. The fragmentation characteristics are determined based on the exact mass information and Rf values. It was found that flavonols, flavones, 1soflavone, chlorogenic acids, amino acids, stilbene and anthocyanins constitute mainly the phytoconstituents of the aerial parts. The biological examination exhibited excellent antioxidant activity using all tested assays and high antibiofilm activity against S. aureus with a reduction value 97.7% at 0.5 mg/ml without affecting bacterial growth which makes these compounds a promising agent for antimicrobial and antibiofilm against bacterial pathogens.